Strawbery DNA

It was very cool to do a little science experiment and to be in contact with another kind of matter different than a circuit board and cables.

It is amazing to me, the meaning of what what we did. Manuela showed me a simple jpg of how DNA was “packed”, and to discover how it was all arranged and how our lab elements were working on that structure….molecules, .. us, ….it is amazing to me.

We did not experiment more than the first try, we were very much happy with the results and that of others that we could see.

Just wondering what to do with the DNA when fished…. so we left it on the alcohol. how would you store it?

What would be a next step now that we did the equivalent of the LED blink? jejeje, it felt so good.

Here is the gallery of the process and a transcript of what the element do at the bottom:

MATERIALS:
Strawberries (at least 2 juicy ones)
Quarter teaspoon of Table salt (NaCl) – don’t use too much of this!
A teaspoon of dishwashing detergent (We used the brand Joy, but any brand will do)
Meat Tenderizer (McCormicks brand, again, any brand should work)
Half a glass of water
Ziplock baggie (1 quart volume)
A disposable coffee filter
A plastic funnel (you may be able to hold the filter some other way in the glass)
Chilled Rubbing alcohol (We used 91% isopropanol, which works better than the 70% strength)
Ice or a fridge to cool the rubbing alcohol prior to using
A tall drinking glass (don’t drink this stuff)
A thin wooden stick (used to fish out your DNA)

PROTOCOL:
Step 1:
 Drop the strawberries into a ziplock baggie.
Step 2: 
Add the detergent, meat tenderizer and salt.
Step 3:
 Seal the baggie and “mechanically disrupt the cells”. Start squishing the cells. Squish and mix them really well with all of the ingredients. Knead the bag for about 5 minutes until you have the consistency of a smoothie
Step 4:
 Open the baggie and add some water. Shake it up a little.
Step 5:
 Place your filter in the funnel and place this in the glass. Now pour the contents of the baggie (the squished strawberry mixture) into the filter.
Step 6: 
Let the contents drip through the filter into the glass. It should look milky pink. Be patient! If the filter is working too slowly, you can gently squeeze it, but not too hard or it may break.
Step 7: 
Holding the glass at an angle, very slowly pour the chilled rubbing alcohol down the side into the strawberry mixture. You want to pour it slow enough so that a layer of the clear alcohol forms on top of the pink layer underneath, since the alcohol is less dense than the water layer containing the strawberries. If you pour too quickly you won’t see this layer form, so remember to do it slowly. Pour enough alcohol so that you have at least a 2 inch layer on top.
Step 8: 
Now set your glass down. You should start to see a wispy/filamentous white layer start to form between the bottom pink layer and the clear alcohol on top. It should take about a minute or more to develop. It will start to look like a small ball of cotton. Sometimes it looks like many small pieces of cotton. Give it some more time and it will gently rise to the surface. This is the strawberry’s DNA starting to precipitate!
Step 9: 
Using your thin wooden stick, you can now fish out the DNA. It will be rather “stringy” since DNA molecules form long chains. These types of molecules are known as “polymers”.

http://j.mp/qEJEPX

Questions and Answers:

Why use strawberries? Strawberries are octoploid, meaning that their cells each have eight duplicate copies of each chromosome. This gives the cells a lot of DNA, which means it is easier to extract a large amount of DNA that is visible to the naked eye.

What’s the table salt for?
DNA is a large molecule that is very negatively charged. Since like charges repel one another, we need to neutralize the charges on the DNA by using the positively charged Sodium ions from salt. This will help the DNA to clump together in the isopropanol.

What about the detergent?
Cells are surrounded by a layer of special lipid molecules. These are very similar to fat. The detergent helps to break apart this protective layer of the cell. This promotes the release of the cells contents, including the DNA.

Meat tenderizer, what’s that for?
Meat tenderizer contains a type of protein called a protease that chops up other proteins. This also helps to release the DNA, since in a cell it is found ‘”wrapped up” with proteins called histones. The protease in meat tenderizer also helps to protect the DNA once it has been released from the cell by chopping up other proteins which in turn chop up DNA. These are called DNAses.

And the 91% rubbing alcohol?
The DNA, in the presence of salt, is not soluble in alcohols, which are weakly polar, such as isopropanol. This is why the DNA forms a visible clump. It prefers to stick to its own molecules rather than mix (dissolve) in the alcohol.

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